Development of 16S rDNA-based PCR assay for detecting Centipeda periodontii and Selenomonas sputigena
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چکیده
منابع مشابه
Selenomonas sputigena septicemia.
Selenomonas sputigena is a curved motile anaerobic gram-negative rod that is a part of the normal upper respiratory tract of man. We present a case of speticemia associated with this organism and believe it to be the first such report describing systemic disease.
متن کاملWhat is broad-range 16S rDNA PCR?
Patel A, et al. Arch Dis Child Educ Pract Ed 2017;0:1–4. doi:10.1136/archdischild-2016-312049 INTRODUCTION PCRs have revolutionised the detection of bacteria in clinical samples since their widespread introduction in the 1990s. Quantitative PCR (qPCR), also known as specific PCR, involves the targeting of particular bacterial species. The technique uses specific primers (short strands of nuclei...
متن کاملDevelopment of broad-range 16S rDNA PCR for use in the routine diagnostic clinical microbiology service.
The aim of the present study was to develop a broad-range PCR based on bacterial 16S rDNA for use in the routine diagnostic clinical microbiology service. The optimization and validation of the assay for use on clinical specimens from normally sterile sites is described, and preliminary results are reported on the use of the assay in the clinical diagnosis of bacterial infection in 382 paediatr...
متن کاملIdentification of acetic acid bacteria by RFLP of PCR-amplified 16S rDNA and 16S-23S rDNA intergenic spacer.
DNA corresponding to 16S rDNA and the 165-23S rDNA intergenic spacer (ITS) from 22 reference strains of acetic acid bacteria, representing the diversity of the family Acetobacteraceae, and 24 indigenous acetic acid bacteria isolated from wine fermentations were analysed by PCR-RFLP. Frateuria aurantia LMG 1558T and Escherichia coli ATCC 11775T were included as outgroups. PCR-amplified products ...
متن کاملApplication of 16S rDNA based seminested PCR for diagnosis of acute bacterial meningitis.
BACKGROUND & OBJECTIVES The diagnosis of bacterial meningitis remains a challenge to the clinician because of its rapid lethal course lacking the consistency to particular clinical signs and symptoms. Moreover, in many clinical settings use of rampant and short course antibiotic therapy prior to lumbar puncture reduces the chance of isolation of bacteria in CSF culture making the diagnosis diff...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2000
ISSN: 0266-8254,1365-2673
DOI: 10.1046/j.1472-765x.2000.00735.x